Starch accumulation was noticed in PIX treated leaves while sucrose content was not changed. The activity of RuBP carboxylase was decreased in PIX treated plants. PIX treated leaves had more chlorophyll content. Net photosynthetic rates were 25% less in PIX-treated leaves. Total leaf area in PIX treated plants was 16% less than the control. The total length of vegetative branches and fruiting branches was 40% and 50% less than the control. PIX was sprayed (0, 7.65, 15.3, 30.6 or 61.2 g active ingredient ha −1) on the plants at first square (25 days after emergence) and measurements were made at frequent intervals. The effects of PIX on plant height, stem elongation, leaf area, net photosynthetic rates, chlorophyll content, sucrose and starch levels, and RuBP carboxylase activity in cotton ( Gossypium hirsutum L. Mepiquat chloride (N, N-dimethylpiperidinium chloride), well known as PIX, is a potential systemic plant growth regulator.
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